Journal: Journal of molecular neuroscience : MN
Article Title: Neuronal Hemoglobin Expression and Its Relevance to Multiple Sclerosis Neuropathology
doi: 10.1007/s12031-015-0711-6
Figure Lengend Snippet: Hbb expression regulates histone H3 trimethylation. a Western blotting shows that the Hbb subunit is expressed in SH-SY5Y cells transfected with pVitro2-Hbb. b Representative Western blot demonstrates that levels of H3K4me3 are increased in nuclear extracts isolated from SH-SY5Y cells transfected with pVitro2-Hbb compared to control cells transfected with vector alone. c Quantitation for H3K4me3 levels in SH-SY5Y cells transfected with either empty vector pVitro2 or pVitro2-Hbb was performed by densitometry from two separate experiments. Error bars represent SEM, *p < 0.05
Article Snippet: The resulting PCR product was cloned into pEGFP (Clontech Laboratories) and then subcloned into the XhoI and NheI sites of the pVitro2 mammalian expression vector (Invivogen, San Diego, CA).
Techniques: Expressing, Western Blot, Transfection, Isolation, Plasmid Preparation, Quantitation Assay